Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HTH_37(antitoxin)
Location 6592586..6593226 Replicon chromosome
Accession NZ_CP118136
Organism Pseudomonas chlororaphis strain NCCB 60038

Toxin (Protein)


Gene name hicA Uniprot ID A0A285GR45
Locus tag PUP52_RS30060 Protein ID WP_028681531.1
Coordinates 6592586..6592768 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PIE8
Locus tag PUP52_RS30065 Protein ID WP_041984783.1
Coordinates 6592801..6593226 (+) Length 142 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUP52_RS30030 (PUP52_30030) 6587905..6588612 + 708 WP_041984770.1 hypothetical protein -
PUP52_RS30035 (PUP52_30035) 6588609..6589532 + 924 WP_041984773.1 hypothetical protein -
PUP52_RS30040 (PUP52_30040) 6589529..6590077 + 549 WP_041984775.1 hypothetical protein -
PUP52_RS30045 (PUP52_30045) 6590074..6590778 + 705 WP_041984777.1 hypothetical protein -
PUP52_RS30050 (PUP52_30050) 6590751..6591722 + 972 WP_041984780.1 hypothetical protein -
PUP52_RS30055 (PUP52_30055) 6591719..6592360 - 642 WP_041985169.1 hypothetical protein -
PUP52_RS30060 (PUP52_30060) 6592586..6592768 + 183 WP_028681531.1 type II toxin-antitoxin system HicA family toxin Toxin
PUP52_RS30065 (PUP52_30065) 6592801..6593226 + 426 WP_041984783.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
PUP52_RS30070 (PUP52_30070) 6593403..6594050 - 648 WP_124355757.1 hypothetical protein -
PUP52_RS30075 (PUP52_30075) 6594892..6595101 + 210 WP_124355758.1 hypothetical protein -
PUP52_RS30080 (PUP52_30080) 6596338..6597141 + 804 WP_080768765.1 lipopolysaccharide biosynthesis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 6545794..6595724 49930


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6764.83 Da        Isoelectric Point: 10.7304

>T272780 WP_028681531.1 NZ_CP118136:6592586-6592768 [Pseudomonas chlororaphis]
MKYSEFRRWLKAQGAEFQPGKGSHFKVTLNGKSTVFPDHGAKEMGEGLRKSIIKQLGLKD

Download         Length: 183 bp

>T272780 NZ_CP118136:6592586-6592768 [Pseudomonas chlororaphis]
ATGAAGTACAGCGAGTTTCGGCGATGGTTGAAAGCCCAAGGCGCGGAGTTCCAACCCGGCAAGGGCAGTCACTTCAAGGT
CACCTTGAATGGCAAGTCAACCGTGTTTCCAGACCACGGAGCCAAGGAAATGGGCGAAGGGTTAAGGAAGTCGATAATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin


Download         Length: 142 a.a.        Molecular weight: 15470.58 Da        Isoelectric Point: 4.4313

>AT272780 WP_041984783.1 NZ_CP118136:6592801-6593226 [Pseudomonas chlororaphis]
MFKYALEIHEEPGTVWLSCEEIPEFHAAGDTVGEALDSVLDALETALSIYVDERRPIPSGNAEAQAGHVLLCLPALTAAK
VALWNAVLDEGTTKAELARRLGIQRPQVDRLLDFLHDSKIENVERALQELGRRISITVEAA

Download         Length: 426 bp

>AT272780 NZ_CP118136:6592801-6593226 [Pseudomonas chlororaphis]
ATGTTCAAGTACGCATTGGAAATTCACGAAGAGCCCGGCACCGTCTGGCTTTCGTGTGAGGAGATTCCAGAATTTCACGC
TGCCGGCGACACCGTAGGCGAGGCGCTGGATAGTGTTTTGGATGCGCTCGAAACGGCTCTGTCTATCTATGTCGATGAAC
GCCGCCCGATTCCATCTGGTAACGCCGAGGCTCAGGCAGGGCACGTTCTGTTATGCCTTCCAGCCCTCACCGCGGCGAAG
GTTGCACTGTGGAACGCGGTGTTGGATGAAGGCACCACCAAGGCAGAGCTGGCGCGCCGCCTGGGGATTCAGCGGCCGCA
GGTGGACCGCTTGCTGGACTTCCTGCACGACTCGAAAATCGAAAACGTCGAGCGCGCCCTGCAGGAACTCGGTCGGCGGA
TCTCTATCACCGTCGAAGCTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285GR45

Antitoxin

Source ID Structure
AlphaFold DB A0A554PIE8

References