Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 6437291..6439020 Replicon chromosome
Accession NZ_CP118135
Organism Pseudomonas chlororaphis strain NCCB 820532

Toxin (Protein)


Gene name pmenT Uniprot ID -
Locus tag PUP76_RS29300 Protein ID WP_274301005.1
Coordinates 6437291..6437935 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID -
Locus tag PUP76_RS29305 Protein ID WP_274301006.1
Coordinates 6437932..6439020 (+) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUP76_RS29285 (PUP76_29285) 6432947..6435322 - 2376 WP_274301002.1 DEAD/DEAH box helicase family protein -
PUP76_RS29290 (PUP76_29290) 6435617..6436537 + 921 WP_274301003.1 restriction endonuclease -
PUP76_RS29295 (PUP76_29295) 6436722..6437294 + 573 WP_274301004.1 DarT ssDNA thymidine ADP-ribosyltransferase family protein -
PUP76_RS29300 (PUP76_29300) 6437291..6437935 + 645 WP_274301005.1 DUF4433 domain-containing protein Toxin
PUP76_RS29305 (PUP76_29305) 6437932..6439020 + 1089 WP_274301006.1 macro domain-containing protein Antitoxin
PUP76_RS29310 (PUP76_29310) 6439358..6440119 + 762 WP_274301007.1 HNH endonuclease -
PUP76_RS29315 (PUP76_29315) 6440442..6441329 - 888 WP_274301008.1 RNA polymerase sigma factor SigJ -
PUP76_RS29320 (PUP76_29320) 6441332..6441772 - 441 WP_062825471.1 carboxymuconolactone decarboxylase family protein -
PUP76_RS29325 (PUP76_29325) 6441873..6442889 - 1017 WP_274301009.1 PDDEXK nuclease domain-containing protein -
PUP76_RS29330 (PUP76_29330) 6443012..6443422 - 411 WP_274301010.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 6417756..6440119 22363


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24816.30 Da        Isoelectric Point: 7.0171

>T272772 WP_274301005.1 NZ_CP118135:6437291-6437935 [Pseudomonas chlororaphis]
MNYSRLNPEKALIWRIVHRDNLPWILDNGLHCASSDVQAPQYVNIGNVDLISKRRSRHVPITPGGVLADYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLYRVEQLGLPFVFTNAHAYPDWTNYYSDLANLSQIDWSILQRRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCHTDAMKECIEQDVTDRGLALPVHARPGWYFQ

Download         Length: 645 bp

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40755.93 Da        Isoelectric Point: 6.8388

>AT272772 WP_274301006.1 NZ_CP118135:6437932-6439020 [Pseudomonas chlororaphis]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFSENYRLYSAACKAGEVETGRMHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWVTEGLHDLRRFLIENQVKSVAVPPLGAGNGGLKWPEVREQIVTVLGDLDVDILVFEPSNQYLNVAKRSG
VEKLTPARALIAELVRRYWILGMECSLLEIQKLAWFLERAIEKLPATENPLNLKFVAHKYGPYANRLEHLLNNLDGSYLH
CNKRISDAGISDVIWFDEERKAFLQTYLKTEAKEYSQALEHTAKLIDGFESPFGMELLATVDWLLSQEGISPTVPAMRDA
LKNWDGGAGAAVRKSRLFDDQALDIALKRLTSSSFMPEMATC

Download         Length: 1089 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References