Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1093288..1093927 Replicon chromosome
Accession NZ_CP118083
Organism Bifidobacterium breve strain VSI11

Toxin (Protein)


Gene name mazF Uniprot ID A0A133L4T2
Locus tag PUW55_RS04860 Protein ID WP_021649636.1
Coordinates 1093288..1093644 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag PUW55_RS04865 Protein ID WP_003829235.1
Coordinates 1093631..1093927 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUW55_RS04835 (PUW55_04835) 1089164..1089946 - 783 WP_021649638.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
PUW55_RS04840 (PUW55_04840) 1090084..1090569 - 486 WP_021649637.1 cytidine deaminase -
PUW55_RS04845 (PUW55_04845) 1090685..1091956 - 1272 WP_065457984.1 IS30 family transposase -
PUW55_RS04850 (PUW55_04850) 1092048..1092404 + 357 WP_012577924.1 SdpI family protein -
PUW55_RS04855 (PUW55_04855) 1092509..1093111 + 603 WP_003829233.1 transglutaminase family protein -
PUW55_RS04860 (PUW55_04860) 1093288..1093644 - 357 WP_021649636.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PUW55_RS04865 (PUW55_04865) 1093631..1093927 - 297 WP_003829235.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
PUW55_RS04870 (PUW55_04870) 1094176..1094850 + 675 WP_015438748.1 histidine phosphatase family protein -
PUW55_RS04875 (PUW55_04875) 1094933..1095316 + 384 WP_016463075.1 DUF948 domain-containing protein -
PUW55_RS04880 (PUW55_04880) 1095316..1095552 + 237 WP_003829239.1 hypothetical protein -
PUW55_RS04885 (PUW55_04885) 1095648..1098326 + 2679 WP_021649635.1 alanine--tRNA ligase -
PUW55_RS04890 (PUW55_04890) 1098335..1098787 + 453 WP_021649634.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1090685..1091956 1271


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(12-54)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13332.27 Da        Isoelectric Point: 8.9603

>T272746 WP_021649636.1 NZ_CP118083:c1093644-1093288 [Bifidobacterium breve]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10997.50 Da        Isoelectric Point: 4.2896

>AT272746 WP_003829235.1 NZ_CP118083:c1093927-1093631 [Bifidobacterium breve]
MMAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTP
QIRDGAVILPEGWDDDED

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133L4T2

Antitoxin

Source ID Structure

References