Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 666726..667834 Replicon chromosome
Accession NZ_CP118080
Organism Streptococcus agalactiae strain VSI14

Toxin (Protein)


Gene name MNTss Uniprot ID A0A3S8RBH6
Locus tag PUW74_RS03600 Protein ID WP_071661597.1
Coordinates 666965..667834 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag PUW74_RS03595 Protein ID WP_000205227.1
Coordinates 666726..666950 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUW74_RS03560 (PUW74_03560) 661942..662112 + 171 WP_004614788.1 cysteine-rich KTR domain-containing protein -
PUW74_RS03565 (PUW74_03565) 662315..662485 + 171 WP_000713595.1 hypothetical protein -
PUW74_RS03570 (PUW74_03570) 662499..663101 + 603 WP_001062586.1 recombinase family protein -
PUW74_RS03575 (PUW74_03575) 663117..663794 + 678 Protein_621 DNA topoisomerase -
PUW74_RS03580 (PUW74_03580) 664150..664602 + 453 WP_223804552.1 DNA recombinase -
PUW74_RS03585 (PUW74_03585) 664603..665019 + 417 WP_000323438.1 recombinase -
PUW74_RS03590 (PUW74_03590) 665039..666583 + 1545 WP_002390960.1 recombinase family protein -
PUW74_RS03595 (PUW74_03595) 666726..666950 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
PUW74_RS03600 (PUW74_03600) 666965..667834 + 870 WP_071661597.1 nucleotidyltransferase domain-containing protein Toxin
PUW74_RS03605 (PUW74_03605) 667815..668549 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
PUW74_RS03610 (PUW74_03610) 668582..669490 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
PUW74_RS03615 (PUW74_03615) 669487..669687 + 201 Protein_629 hypothetical protein -
PUW74_RS03620 (PUW74_03620) 669767..670929 + 1163 WP_088186165.1 IS3-like element ISSag12 family transposase -
PUW74_RS03625 (PUW74_03625) 671088..671339 + 252 Protein_631 GNAT family N-acetyltransferase -
PUW74_RS03630 (PUW74_03630) 671432..672226 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element tet(O) / ant(6)-Ia / aph(3')-III / erm(B) gbs0628 / gbs0630 605961..712193 106232


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32902.55 Da        Isoelectric Point: 4.9770

>T272744 WP_071661597.1 NZ_CP118080:666965-667834 [Streptococcus agalactiae]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT272744 WP_000205227.1 NZ_CP118080:666726-666950 [Streptococcus agalactiae]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S8RBH6

Antitoxin

Source ID Structure

References