Detailed information of TA system TA027273

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4206590..4207381
• Replicon: chromosome
• Accession: NC_017631
• Organism: Escherichia coli ABU 83972

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: ECABU_RS20855
• Protein ID: WP_000691791.1
• Coordinates: 4206956..4207381 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: ECABU_RS20850
• Protein ID: WP_000939437.1
• Coordinates: 4206590..4206925 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECABU_RS20825	4202537..4202755	-	219	WP_000070772.1	AlpA family phage regulatory protein	-
ECABU_RS20830	4202873..4203487	-	615	WP_000772910.1	inovirus Gp2 family protein	-
ECABU_RS20835	4204080..4205033	+	954	WP_000290406.1	hypothetical protein	-
ECABU_RS20840	4205269..4206087	+	819	WP_000761990.1	DUF945 domain-containing protein	-
ECABU_RS20845	4206117..4206590	+	474	WP_001292742.1	DNA repair protein RadC	-
ECABU_RS20850	4206590..4206925	+	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECABU_RS20855	4206956..4207381	+	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
ECABU_RS20860	4207402..4208593	+	1192	Protein_3957	AAA family ATPase	-
ECABU_RS28800	4208578..4208799	+	222	WP_126125326.1	hypothetical protein	-
ECABU_RS20865	4208796..4209527	+	732	WP_001216639.1	TIGR02646 family protein	-
ECABU_RS20875	4210150..4210536	+	387	WP_001270938.1	DUF2441 domain-containing protein	-
ECABU_RS20885	4211218..4211715	+	498	WP_000509808.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4185924..4211715	25791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T27273 WP_000691791.1 NC_017631:4206956-4207381 [Escherichia coli ABU 83972]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T27273 NC_017631:4206956-4207381 [Escherichia coli ABU 83972]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT27273 WP_000939437.1 NC_017631:4206590-4206925 [Escherichia coli ABU 83972]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT27273 NC_017631:4206590-4206925 [Escherichia coli ABU 83972]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6