Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2536114..2536765 Replicon chromosome
Accession NZ_CP118050
Organism Lacticaseibacillus rhamnosus strain VSI43

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag PUW66_RS11705 Protein ID WP_005686631.1
Coordinates 2536114..2536497 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag PUW66_RS11710 Protein ID WP_005686632.1
Coordinates 2536517..2536765 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUW66_RS11700 (PUW66_11700) 2535176..2535703 - 528 WP_049152640.1 QueT transporter family protein -
PUW66_RS11705 (PUW66_11705) 2536114..2536497 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PUW66_RS11710 (PUW66_11710) 2536517..2536765 - 249 WP_005686632.1 antitoxin Antitoxin
PUW66_RS11715 (PUW66_11715) 2536877..2538016 - 1140 WP_005691215.1 alanine racemase -
PUW66_RS11720 (PUW66_11720) 2538003..2538377 - 375 WP_005686634.1 holo-ACP synthase -
PUW66_RS11725 (PUW66_11725) 2538546..2540054 - 1509 WP_005686635.1 DEAD/DEAH box helicase -
PUW66_RS11730 (PUW66_11730) 2540328..2541716 - 1389 WP_014571607.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2523247..2594233 70986


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T272727 WP_005686631.1 NZ_CP118050:c2536497-2536114 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT272727 WP_005686632.1 NZ_CP118050:c2536765-2536517 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References