Detailed information of TA system TA272721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2309681..2310720
• Replicon: chromosome
• Accession: NZ_CP118048
• Organism: Enterococcus faecalis strain VSI48

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: PUW76_RS11005
• Protein ID: WP_010824179.1
• Coordinates: 2310061..2310720 (+)
• Length: 220 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: PUW76_RS11000
• Protein ID: WP_010824178.1
• Coordinates: 2309681..2310064 (+)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PUW76_RS10945 (2305509)	2305509..2305877	-	369	WP_002365216.1	DUF86 domain-containing protein	-
PUW76_RS10950 (2305861)	2305861..2306193	-	333	WP_002399122.1	nucleotidyltransferase domain-containing protein	-
PUW76_RS10955 (2306253)	2306253..2306576	-	324	WP_002399121.1	hypothetical protein	-
PUW76_RS10960 (2306616)	2306616..2306777	-	162	WP_229236153.1	hypothetical protein	-
PUW76_RS10965 (2306866)	2306866..2307018	-	153	WP_002399525.1	hypothetical protein	-
PUW76_RS10970 (2307045)	2307045..2307269	-	225	WP_010824177.1	hypothetical protein	-
PUW76_RS10975 (2307501)	2307501..2307689	+	189	WP_002365223.1	hypothetical protein	-
PUW76_RS10980 (2307676)	2307676..2307837	-	162	WP_002365224.1	hypothetical protein	-
PUW76_RS10985 (2307860)	2307860..2308276	-	417	WP_002408315.1	DUF961 family protein	-
PUW76_RS10990 (2308276)	2308276..2308602	-	327	WP_010710084.1	hypothetical protein	-
PUW76_RS10995 (2308688)	2308688..2308939	-	252	WP_010777935.1	hypothetical protein	-
PUW76_RS11000 (2309681)	2309681..2310064	+	384	WP_010824178.1	helix-turn-helix transcriptional regulator	Antitoxin
PUW76_RS11005 (2310061)	2310061..2310720	+	660	WP_010824179.1	ImmA/IrrE family metallo-endopeptidase	Toxin
PUW76_RS11010 (2310736)	2310736..2311071	+	336	WP_010824180.1	helix-turn-helix domain-containing protein	-
PUW76_RS11015 (2311109)	2311109..2312440	-	1332	WP_010824181.1	FAD-dependent oxidoreductase	-
PUW76_RS11020 (2312540)	2312540..2313475	-	936	WP_174115432.1	aldo/keto reductase	-
PUW76_RS11025 (2313490)	2313490..2313909	-	420	WP_002365234.1	MerR family transcriptional regulator	-
PUW76_RS11030 (2313926)	2313926..2314507	-	582	WP_002380883.1	histidine phosphatase family protein	-
PUW76_RS11035 (2314729)	2314729..2315058	+	330	WP_010816770.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2270907..2317241	46334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (80-181)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 220 a.a.
• Molecular weight: 26320.31 Da
• Isoelectric Point: 7.0496

>T272721 WP_010824179.1 NZ_CP118048:2310061-2310720 [Enterococcus faecalis]
MIGLLWVDFISKKLYFEAFNFSNKLIKEVSYYSSKNIEQVKCFDIEKYVKEVEDVEFVEYTFQKQLKRRMLGSISKVDDE
VIITTNKELMLERKNFTKMHEVMHYYIDIPKINNATHTFSDILLKNGYLMEDFPKEYRANVGASMLMANDQALFYALKKF
YSFEEIAQYFFMSKSALRNRLIEHLIYVNNCTFAHANTLFNNYYFHKETDIYRFIFNDS

Nucleotide

• Length: 660 bp

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 15032.08 Da
• Isoelectric Point: 5.3750

>AT272721 WP_010824178.1 NZ_CP118048:2309681-2310064 [Enterococcus faecalis]
VQPLAKRLQLLREEKEWTKTYVAKQLGLNNLGTYANWEYGTREPDSEMLSKIASLYDVSTDFLLGRTEERIHLNDKEKIG
KNIISHFRLNTSDMDIEDIEELEEELIDFQDFLIKKAKEKKKRNKKD

Nucleotide

• Length: 384 bp