Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2599761..2600412 Replicon chromosome
Accession NZ_CP118035
Organism Lacticaseibacillus rhamnosus strain VSI33

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag PUW67_RS12065 Protein ID WP_005686631.1
Coordinates 2599761..2600144 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag PUW67_RS12070 Protein ID WP_005686632.1
Coordinates 2600164..2600412 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUW67_RS12060 (PUW67_12060) 2598720..2599247 - 528 WP_005691221.1 QueT transporter family protein -
PUW67_RS12065 (PUW67_12065) 2599761..2600144 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PUW67_RS12070 (PUW67_12070) 2600164..2600412 - 249 WP_005686632.1 antitoxin Antitoxin
PUW67_RS12075 (PUW67_12075) 2600524..2601663 - 1140 WP_005691215.1 alanine racemase -
PUW67_RS12080 (PUW67_12080) 2601650..2602024 - 375 WP_005686634.1 holo-ACP synthase -
PUW67_RS12085 (PUW67_12085) 2602193..2603701 - 1509 WP_005686635.1 DEAD/DEAH box helicase -
PUW67_RS12090 (PUW67_12090) 2603975..2605363 - 1389 WP_014571607.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T272700 WP_005686631.1 NZ_CP118035:c2600144-2599761 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT272700 WP_005686632.1 NZ_CP118035:c2600412-2600164 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References