Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1477093..1477729 Replicon chromosome
Accession NZ_CP118021
Organism Bacillus spizizenii strain B-354

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PT671_RS07205 Protein ID WP_003156187.1
Coordinates 1477093..1477443 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag PT671_RS07210 Protein ID WP_003225183.1
Coordinates 1477448..1477729 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT671_RS07165 1472134..1472733 - 600 WP_003225202.1 phosphoserine phosphatase RsbX -
PT671_RS07170 1472733..1473521 - 789 WP_003225200.1 RNA polymerase sigma factor SigB -
PT671_RS07175 1473487..1473969 - 483 WP_003225198.1 anti-sigma B factor RsbW -
PT671_RS07180 1473966..1474295 - 330 WP_003225196.1 anti-sigma factor antagonist RsbV -
PT671_RS07185 1474357..1475364 - 1008 WP_003225194.1 phosphoserine phosphatase RsbU -
PT671_RS07190 1475376..1475777 - 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
PT671_RS07195 1475781..1476146 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
PT671_RS07200 1476151..1476975 - 825 WP_003225186.1 RsbT co-antagonist protein RsbRA -
PT671_RS07205 1477093..1477443 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PT671_RS07210 1477448..1477729 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PT671_RS07215 1477845..1479014 - 1170 WP_003225181.1 alanine racemase -
PT671_RS07220 1479129..1480145 - 1017 WP_072181690.1 outer membrane lipoprotein carrier protein LolA -
PT671_RS07225 1480310..1480675 - 366 WP_003225177.1 holo-ACP synthase -
PT671_RS07230 1480770..1481369 + 600 WP_003225175.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T272694 WP_003156187.1 NZ_CP118021:c1477443-1477093 [Bacillus spizizenii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT272694 WP_003225183.1 NZ_CP118021:c1477729-1477448 [Bacillus spizizenii]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References