Detailed information of TA system TA272652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 483875..485222
• Replicon: chromosome
• Accession: NZ_CP117989
• Organism: Vibrio campbellii strain 20220413_1

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: PUN50_RS19060
• Protein ID: WP_274291884.1
• Coordinates: 484281..485222 (+)
• Length: 314 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: PUN50_RS19055
• Protein ID: WP_061007692.1
• Coordinates: 483875..484279 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PUN50_RS19040 (PUN50_19040)	481538..481990	-	453	WP_021018079.1	hypothetical protein	-
PUN50_RS19045 (PUN50_19045)	482571..482813	+	243	WP_061007694.1	helix-turn-helix domain-containing protein	-
PUN50_RS19050 (PUN50_19050)	483093..483686	+	594	Protein_426	tyrosine-type recombinase/integrase	-
PUN50_RS19055 (PUN50_19055)	483875..484279	+	405	WP_061007692.1	DUF6088 family protein	Antitoxin
PUN50_RS19060 (PUN50_19060)	484281..485222	+	942	WP_274291884.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
PUN50_RS19065 (PUN50_19065)	485242..486759	+	1518	WP_274291885.1	type I restriction-modification system subunit M	-
PUN50_RS19070 (PUN50_19070)	486749..487921	+	1173	WP_274291886.1	restriction endonuclease subunit S	-
PUN50_RS19075 (PUN50_19075)	487987..488823	+	837	WP_061007688.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (37-300)

Antitoxin

• Protein of unknown function DUF6088 (7-130)

Sequences

Toxin

Protein

• Length: 314 a.a.
• Molecular weight: 35760.27 Da
• Isoelectric Point: 5.9416

>T272652 WP_274291884.1 NZ_CP117989:484281-485222 [Vibrio campbellii]
MNTLKQQFLEVSDALELGNPAIIEKDYWVVALLAELAKVCPEHHQMVFSGGTALAKSNVKILRMSEDVDIKLIPNPAFDE
LSRAKKKATRKACVNKIEQALTQSGRFNVETRSVRDEYRYVEFELKYPQQFSQAPCLRPIIKLELIETIPLLDVESRSIQ
SLVAELYQQPQEVSAFDCVSIHATLVEKIISMLRRTMSVKRNTERSDDVALVRHIYDVHCITKLSMPELDSIDGLFKAVL
AEDIARFGNQHAEFVANPKQELQLGLKELEENAIFRQRFQEFVTPMVFNTEPHDFDTCFASFKRIAASLIEKI

Nucleotide

• Length: 942 bp

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15154.52 Da
• Isoelectric Point: 10.4983

>AT272652 WP_061007692.1 NZ_CP117989:483875-484279 [Vibrio campbellii]
MTAVDRICQKIKRSRRYVFERKDFDGFASYDQIGRALRQLVQQGELMKLGYGLYTKARINSLTGKPMPTNPGGTDALMRE
ILKMKGVDFEMDSLSLQSLSGSSTQIPSSIQYSWDPKQFNRKLVVGSRVLNLPS

Nucleotide

• Length: 405 bp