Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4431324..4431900 Replicon chromosome
Accession NZ_CP117971
Organism Escherichia coli strain B-1109

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag PT723_RS21810 Protein ID WP_001299718.1
Coordinates 4431613..4431900 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0E2KXJ8
Locus tag PT723_RS21805 Protein ID WP_000063159.1
Coordinates 4431324..4431626 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT723_RS21790 (4427913) 4427913..4430063 + 2151 WP_001299714.1 pyruvate/proton symporter BtsT -
PT723_RS21795 (4430113) 4430113..4430316 + 204 WP_000467859.1 YbdD/YjiX family protein -
PT723_RS21800 (4430327) 4430327..4431283 + 957 WP_001299717.1 GTPase -
PT723_RS21805 (4431324) 4431324..4431626 - 303 WP_000063159.1 BrnA antitoxin family protein Antitoxin
PT723_RS21810 (4431613) 4431613..4431900 - 288 WP_001299718.1 BrnT family toxin Toxin
PT723_RS21815 (4432097) 4432097..4432780 + 684 Protein_4250 restriction endonuclease -
PT723_RS21820 (4432771) 4432771..4434693 + 1923 Protein_4251 DUF3387 domain-containing protein -
PT723_RS21825 (4434908) 4434908..4435246 + 339 WP_000132639.1 endoribonuclease SymE -
PT723_RS21830 (4435334) 4435334..4435486 - 153 WP_229018381.1 cytoplasmic protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T272607 WP_001299718.1 NZ_CP117971:c4431900-4431613 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11536.01 Da        Isoelectric Point: 9.4646

>AT272607 WP_000063159.1 NZ_CP117971:c4431626-4431324 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2KXJ8

References