Detailed information of TA system TA272580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 229360..229931
• Replicon: chromosome
• Accession: NZ_CP117970
• Organism: Enterococcus faecalis strain B-537

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: PT722_RS01035
• Protein ID: WP_002354774.1
• Coordinates: 229360..229701 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: PT722_RS01040
• Protein ID: WP_002354773.1
• Coordinates: 229701..229931 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PT722_RS01030 (225375)	225375..228989	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
PT722_RS01035 (229360)	229360..229701	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PT722_RS01040 (229701)	229701..229931	-	231	WP_002354773.1	hypothetical protein	Antitoxin
PT722_RS01045 (230338)	230338..230553	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
PT722_RS01050 (230692)	230692..231684	+	993	WP_002365428.1	biotin--[acetyl-CoA-carboxylase] ligase	-
PT722_RS01055 (231751)	231751..232383	-	633	WP_002358972.1	RloB family protein	-
PT722_RS01060 (232392)	232392..233687	-	1296	WP_002371641.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T272580 WP_002354774.1 NZ_CP117970:c229701-229360 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T272580 NZ_CP117970:c229701-229360 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT272580 WP_002354773.1 NZ_CP117970:c229931-229701 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT272580 NZ_CP117970:c229931-229701 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812