Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3117146..3117800 Replicon chromosome
Accession NZ_CP117962
Organism Escherichia coli strain JM109

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag PT299_RS16360 Protein ID WP_000244777.1
Coordinates 3117393..3117800 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag PT299_RS16355 Protein ID WP_000354046.1
Coordinates 3117146..3117412 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT299_RS16330 (3112315) 3112315..3113058 + 744 WP_000951964.1 SDR family oxidoreductase -
PT299_RS16335 (3113115) 3113115..3114548 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
PT299_RS16340 (3114593) 3114593..3114904 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
PT299_RS16345 (3115068) 3115068..3115727 + 660 WP_000250274.1 hemolysin III family protein -
PT299_RS16350 (3115923) 3115923..3116903 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
PT299_RS16355 (3117146) 3117146..3117412 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
PT299_RS16360 (3117393) 3117393..3117800 + 408 WP_000244777.1 protein YgfX Toxin
PT299_RS16365 (3117840) 3117840..3118361 - 522 WP_001055874.1 flavodoxin FldB -
PT299_RS16370 (3118473) 3118473..3119369 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
PT299_RS16375 (3119394) 3119394..3120104 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
PT299_RS16380 (3120110) 3120110..3121843 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T272554 WP_000244777.1 NZ_CP117962:3117393-3117800 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp


Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT272554 WP_000354046.1 NZ_CP117962:3117146-3117412 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7


Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References