Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 154898..155523 Replicon plasmid unnamed1
Accession NZ_CP117961
Organism Escherichia coli strain JM109

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag PT299_RS00860 Protein ID WP_000911324.1
Coordinates 154898..155296 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID L4J1D2
Locus tag PT299_RS00865 Protein ID WP_000450532.1
Coordinates 155296..155523 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT299_RS00845 (151206) 151206..151727 + 522 WP_000632670.1 conjugal transfer entry exclusion protein TraS -
PT299_RS00850 (151752) 151752..152483 + 732 WP_000850422.1 conjugal transfer complement resistance protein TraT -
PT299_RS00855 (152736) 152736..154889 + 2154 WP_053905128.1 type IV conjugative transfer system coupling protein TraD -
PT299_RS00860 (154898) 154898..155296 - 399 WP_000911324.1 type II toxin-antitoxin system VapC family toxin Toxin
PT299_RS00865 (155296) 155296..155523 - 228 WP_000450532.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC 1..231544 231544


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14800.09 Da        Isoelectric Point: 7.8604

>T272539 WP_000911324.1 NZ_CP117961:c155296-154898 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8512.55 Da        Isoelectric Point: 4.5358

>AT272539 WP_000450532.1 NZ_CP117961:c155523-155296 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CHF5

References