272534

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 11036..11715 Replicon plasmid unnamed1
Accession NZ_CP117961
Organism Escherichia coli strain JM109

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag PT299_RS00075 Protein ID WP_000854672.1
Coordinates 11036..11377 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag PT299_RS00080 Protein ID WP_000070395.1
Coordinates 11398..11715 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT299_RS00050 (6313) 6313..6714 + 402 WP_000174689.1 sigma factor-binding protein Crl -
PT299_RS00055 (6753) 6753..7808 - 1056 WP_000749863.1 phosphoporin PhoE -
PT299_RS00060 (8096) 8096..9199 + 1104 WP_001285288.1 glutamate 5-kinase -
PT299_RS00065 (9211) 9211..10464 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
PT299_RS00075 (11036) 11036..11377 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
PT299_RS00080 (11398) 11398..11715 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
PT299_RS00085 (11734) 11734..11955 - 222 WP_000691994.1 DUF987 domain-containing protein -
PT299_RS00090 (11964) 11964..12440 - 477 WP_000811693.1 RadC family protein -
PT299_RS00095 (12456) 12456..12914 - 459 WP_000211838.1 antirestriction protein -
PT299_RS00100 (13012) 13012..13251 - 240 WP_000194654.1 DUF905 family protein -
PT299_RS00105 (13328) 13328..13795 - 468 WP_001547765.1 protein YkfB -
PT299_RS00110 (13818) 13818..14261 - 444 WP_000824223.1 lipoprotein YafY -
PT299_RS00115 (14261) 14261..14488 - 228 WP_001548158.1 protein YpjK -
PT299_RS00120 (14484) 14484..14675 - 192 Protein_22 DeoR family transcriptional regulator -
PT299_RS00125 (14892) 14892..15713 - 822 WP_000197389.1 DUF932 domain-containing protein -
PT299_RS00130 (15805) 15805..16668 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC 1..231544 231544


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T272534 WP_000854672.1 NZ_CP117961:c11377-11036 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT272534 WP_000070395.1 NZ_CP117961:c11715-11398 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References