Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2200238..2200735 Replicon chromosome
Accession NZ_CP117960
Organism Escherichia coli strain B-3008

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PT288_RS10775 Protein ID WP_001173343.1
Coordinates 2200238..2200411 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PT288_RS10780 Protein ID WP_000398619.1
Coordinates 2200439..2200735 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT288_RS10765 (2196908) 2196908..2198365 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
PT288_RS10770 (2198602) 2198602..2200119 + 1518 WP_001295087.1 lysine--tRNA ligase -
PT288_RS10775 (2200238) 2200238..2200411 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PT288_RS10780 (2200439) 2200439..2200735 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PT288_RS10785 (2200962) 2200962..2201234 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
PT288_RS10790 (2201246) 2201246..2201476 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
PT288_RS10795 (2201657) 2201657..2203288 + 1632 WP_001216477.1 sensor histidine kinase -
PT288_RS10800 (2203285) 2203285..2204004 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2184759..2200735 15976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T272523 WP_001173343.1 NZ_CP117960:c2200411-2200238 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT272523 WP_000398619.1 NZ_CP117960:c2200735-2200439 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References