Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 63335..63860 Replicon plasmid pUM146
Accession NC_017630
Organism Escherichia coli UM146

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag UM146_RS25115 Protein ID WP_001159871.1
Coordinates 63335..63640 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag UM146_RS25120 Protein ID WP_000813634.1
Coordinates 63642..63860 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UM146_RS28905 58602..59873 + 1272 Protein_74 translesion error-prone DNA polymerase V subunit UmuC -
UM146_RS25090 59878..60270 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
UM146_RS25095 60275..61246 - 972 WP_001103694.1 hypothetical protein -
UM146_RS25100 61475..62119 + 645 WP_000633911.1 AAA family ATPase -
UM146_RS25105 62113..62388 + 276 WP_000239529.1 hypothetical protein -
UM146_RS25110 62526..63334 - 809 Protein_79 phage integrase family protein -
UM146_RS25115 63335..63640 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
UM146_RS25120 63642..63860 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
UM146_RS25125 66182..66823 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114550 114550


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T27252 WP_001159871.1 NC_017630:c63640-63335 [Escherichia coli UM146]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T27252 NC_017630:c63640-63335 [Escherichia coli UM146]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT27252 WP_000813634.1 NC_017630:c63860-63642 [Escherichia coli UM146]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT27252 NC_017630:c63860-63642 [Escherichia coli UM146]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References