Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4689258..4689834 Replicon chromosome
Accession NZ_CP117959
Organism Escherichia coli strain B-2207

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag PT284_RS23470 Protein ID WP_001297643.1
Coordinates 4689258..4689545 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag PT284_RS23475 Protein ID WP_000063156.1
Coordinates 4689532..4689834 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT284_RS23455 (4685495) 4685495..4686775 - 1281 WP_001037390.1 DUF445 domain-containing protein -
PT284_RS23460 (4686816) 4686816..4688048 - 1233 WP_001137032.1 multidrug efflux MFS transporter MdtM -
PT284_RS23465 (4688363) 4688363..4689059 + 697 Protein_4435 IS1 family transposase -
PT284_RS23470 (4689258) 4689258..4689545 + 288 WP_001297643.1 BrnT family toxin Toxin
PT284_RS23475 (4689532) 4689532..4689834 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
PT284_RS23480 (4689868) 4689868..4690824 - 957 WP_001297640.1 GTPase -
PT284_RS23485 (4690835) 4690835..4691038 - 204 WP_000467860.1 YbdD/YjiX family protein -
PT284_RS23490 (4691088) 4691088..4693238 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4688850..4689059 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T272511 WP_001297643.1 NZ_CP117959:4689258-4689545 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT272511 WP_000063156.1 NZ_CP117959:4689532-4689834 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References