Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4776968..4777465 Replicon chromosome
Accession NC_017628
Organism Escherichia coli IHE3034

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECOK1_RS23935 Protein ID WP_001173343.1
Coordinates 4777292..4777465 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag ECOK1_RS23930 Protein ID WP_001312331.1
Coordinates 4776968..4777264 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOK1_RS23910 4773697..4774416 - 720 WP_000611291.1 two-component system response regulator DcuR -
ECOK1_RS23915 4774413..4776044 - 1632 WP_001216482.1 sensor histidine kinase -
ECOK1_RS23920 4776225..4776455 + 231 WP_000373293.1 (4Fe-4S)-binding protein -
ECOK1_RS23925 4776467..4776739 + 273 WP_000405647.1 N-acetyltransferase -
ECOK1_RS23930 4776968..4777264 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECOK1_RS23935 4777292..4777465 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECOK1_RS23940 4777584..4779101 - 1518 WP_000003806.1 lysine--tRNA ligase -
ECOK1_RS23945 4779338..4780795 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T27251 WP_001173343.1 NC_017628:4777292-4777465 [Escherichia coli IHE3034]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T27251 NC_017628:4777292-4777465 [Escherichia coli IHE3034]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT27251 WP_001312331.1 NC_017628:4776968-4777264 [Escherichia coli IHE3034]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT27251 NC_017628:4776968-4777264 [Escherichia coli IHE3034]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References