Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4354739..4355236 Replicon chromosome
Accession NZ_CP117959
Organism Escherichia coli strain B-2207

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PT284_RS21795 Protein ID WP_001173343.1
Coordinates 4355063..4355236 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PT284_RS21790 Protein ID WP_000398619.1
Coordinates 4354739..4355035 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT284_RS21770 (4351470) 4351470..4352189 - 720 WP_000611288.1 two-component system response regulator DcuR -
PT284_RS21775 (4352186) 4352186..4353817 - 1632 WP_066009715.1 sensor histidine kinase -
PT284_RS21780 (4353998) 4353998..4354228 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
PT284_RS21785 (4354240) 4354240..4354512 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
PT284_RS21790 (4354739) 4354739..4355035 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PT284_RS21795 (4355063) 4355063..4355236 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PT284_RS21800 (4355355) 4355355..4356872 - 1518 WP_078046142.1 lysine--tRNA ligase -
PT284_RS21805 (4357109) 4357109..4358566 - 1458 WP_066009710.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4354739..4400779 46040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T272509 WP_001173343.1 NZ_CP117959:4355063-4355236 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT272509 WP_000398619.1 NZ_CP117959:4354739-4355035 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References