272480

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4071..4596 Replicon plasmid unnamed1
Accession NZ_CP117958
Organism Escherichia coli strain B-2207

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag PT284_RS00020 Protein ID WP_001159868.1
Coordinates 4071..4376 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag PT284_RS00025 Protein ID WP_000813634.1
Coordinates 4378..4596 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT284_RS00010 (1735) 1735..2490 - 756 WP_000852146.1 replication initiation protein RepE -
PT284_RS00015 (3264) 3264..4070 - 807 WP_000016982.1 site-specific integrase -
PT284_RS00020 (4071) 4071..4376 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
PT284_RS00025 (4378) 4378..4596 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
PT284_RS00030 (5188) 5188..5676 + 489 WP_011254646.1 hypothetical protein -
PT284_RS00035 (5710) 5710..6843 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
PT284_RS00040 (7010) 7010..7783 - 774 WP_000905949.1 hypothetical protein -
PT284_RS00045 (7796) 7796..8296 - 501 WP_102360530.1 HEPN family nuclease -
PT284_RS00050 (8561) 8561..8791 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
PT284_RS00055 (8788) 8788..9204 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iucA / iucB / iucB / iucC / iucD / iutA 1..122293 122293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T272480 WP_001159868.1 NZ_CP117958:c4376-4071 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T272480 NZ_CP117958:c4376-4071 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT272480 WP_000813634.1 NZ_CP117958:c4596-4378 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT272480 NZ_CP117958:c4596-4378 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References