272473

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 477250..477887 Replicon chromosome
Accession NZ_CP117945
Organism Bacillus velezensis strain ZY1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PT966_RS02480 Protein ID WP_003156187.1
Coordinates 477537..477887 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PT966_RS02475 Protein ID WP_003156188.1
Coordinates 477250..477531 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT966_RS02455 (PT966_02455) 473615..474214 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
PT966_RS02460 (PT966_02460) 474307..474672 + 366 WP_003156192.1 holo-ACP synthase -
PT966_RS02465 (PT966_02465) 474837..475844 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
PT966_RS02470 (PT966_02470) 475961..477130 + 1170 WP_025853726.1 alanine racemase -
PT966_RS02475 (PT966_02475) 477250..477531 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PT966_RS02480 (PT966_02480) 477537..477887 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PT966_RS02485 (PT966_02485) 478005..478826 + 822 WP_014304404.1 STAS domain-containing protein -
PT966_RS02490 (PT966_02490) 478831..479196 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
PT966_RS02495 (PT966_02495) 479199..479600 + 402 WP_003156178.1 anti-sigma regulatory factor -
PT966_RS02500 (PT966_02500) 479612..480619 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
PT966_RS02505 (PT966_02505) 480683..481012 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
PT966_RS02510 (PT966_02510) 481009..481491 + 483 WP_003156173.1 anti-sigma B factor RsbW -
PT966_RS02515 (PT966_02515) 481457..482245 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
PT966_RS02520 (PT966_02520) 482245..482847 + 603 WP_270355603.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T272473 WP_003156187.1 NZ_CP117945:477537-477887 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT272473 WP_003156188.1 NZ_CP117945:477250-477531 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References