Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 526504..527141 Replicon chromosome
Accession NZ_CP117857
Organism Bacillus velezensis strain PT4

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PO845_RS02575 Protein ID WP_003156187.1
Coordinates 526791..527141 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PO845_RS02570 Protein ID WP_003156188.1
Coordinates 526504..526785 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PO845_RS02550 (PO845_02550) 522869..523468 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
PO845_RS02555 (PO845_02555) 523561..523926 + 366 WP_053104569.1 holo-ACP synthase -
PO845_RS02560 (PO845_02560) 524091..525098 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
PO845_RS02565 (PO845_02565) 525215..526384 + 1170 WP_039252648.1 alanine racemase -
PO845_RS02570 (PO845_02570) 526504..526785 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PO845_RS02575 (PO845_02575) 526791..527141 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PO845_RS02580 (PO845_02580) 527259..528080 + 822 WP_280955859.1 STAS domain-containing protein -
PO845_RS02585 (PO845_02585) 528085..528450 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
PO845_RS02590 (PO845_02590) 528453..528854 + 402 WP_003156178.1 anti-sigma regulatory factor -
PO845_RS02595 (PO845_02595) 528866..529873 + 1008 WP_280955860.1 PP2C family protein-serine/threonine phosphatase -
PO845_RS02600 (PO845_02600) 529937..530266 + 330 WP_033575044.1 anti-sigma factor antagonist -
PO845_RS02605 (PO845_02605) 530263..530745 + 483 WP_007609591.1 anti-sigma B factor RsbW -
PO845_RS02610 (PO845_02610) 530711..531499 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
PO845_RS02615 (PO845_02615) 531499..532101 + 603 WP_039252641.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T272421 WP_003156187.1 NZ_CP117857:526791-527141 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT272421 WP_003156188.1 NZ_CP117857:526504-526785 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References