272414

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2019005..2019595 Replicon chromosome
Accession NZ_CP117853
Organism Klebsiella michiganensis strain 2020CK-00215

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag PUO96_RS09570 Protein ID WP_008804165.1
Coordinates 2019263..2019595 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag PUO96_RS09565 Protein ID WP_064405693.1
Coordinates 2019005..2019262 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUO96_RS09550 (PUO96_09550) 2016330..2017823 - 1494 WP_274229442.1 hypothetical protein -
PUO96_RS09555 (PUO96_09555) 2017895..2018284 - 390 WP_274229443.1 hypothetical protein -
PUO96_RS09560 (PUO96_09560) 2018541..2018744 + 204 WP_224243742.1 helix-turn-helix domain-containing protein -
PUO96_RS09565 (PUO96_09565) 2019005..2019262 + 258 WP_064405693.1 hypothetical protein Antitoxin
PUO96_RS09570 (PUO96_09570) 2019263..2019595 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PUO96_RS09580 (PUO96_09580) 2019919..2021376 + 1458 WP_025106072.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
PUO96_RS09590 (PUO96_09590) 2022338..2023792 - 1455 WP_014229909.1 AMP nucleosidase -
PUO96_RS09595 (PUO96_09595) 2023925..2024182 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2001863..2019595 17732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T272414 WP_008804165.1 NZ_CP117853:2019263-2019595 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9452.97 Da        Isoelectric Point: 4.4607

>AT272414 WP_064405693.1 NZ_CP117853:2019005-2019262 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSMDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure

References