Detailed information of TA system TA272328

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 930358..931073
• Replicon: chromosome
• Accession: NZ_CP117838
• Organism: Enterobacter sp. S-33

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: PUP35_RS04345
• Protein ID: WP_274242181.1
• Coordinates: 930705..931073 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: PUP35_RS04340
• Protein ID: WP_274242180.1
• Coordinates: 930358..930684 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PUP35_RS04315	926226..927116	+	891	WP_274242175.1	50S ribosome-binding GTPase	-
PUP35_RS04320	927422..927973	+	552	WP_274242176.1	hypothetical protein	-
PUP35_RS04325	927979..928605	+	627	WP_274242177.1	hypothetical protein	-
PUP35_RS04330	928987..929805	+	819	WP_274242178.1	DUF932 domain-containing protein	-
PUP35_RS04335	929875..930345	+	471	WP_274242179.1	DNA repair protein RadC	-
PUP35_RS04340	930358..930684	+	327	WP_274242180.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
PUP35_RS04345	930705..931073	+	369	WP_274242181.1	TA system toxin CbtA family protein	Toxin
PUP35_RS04350	931757..934066	+	2310	WP_274242182.1	TerB N-terminal domain-containing protein	-
PUP35_RS04355	934070..935386	+	1317	WP_166876384.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	909802..950126	40324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13797.22 Da
• Isoelectric Point: 9.5668

>T272328 WP_274242181.1 NZ_CP117838:930705-931073 [Enterobacter sp. S-33]
MKFLPATNMRAAKPCLTPVTIWQMLLTRLLKQHYGLMLSDTPFSDEAVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQLQAPYLTATDILHARKACGLMARCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12080.80 Da
• Isoelectric Point: 6.7384

>AT272328 WP_274242180.1 NZ_CP117838:930358-930684 [Enterobacter sp. S-33]
MSLIPAHEWGIKSDIVPRLGARLIQEGSQLHYLADRAGITGKFSDAECLKLNVAFPHFIRQMESMLSSGEINPHHARCVT
LYHNDFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 327 bp