Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5330390..5331015 Replicon chromosome
Accession NZ_CP117745
Organism Klebsiella pneumoniae strain 2022CK-00752

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag PTC85_RS26090 Protein ID WP_002882817.1
Coordinates 5330390..5330773 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag PTC85_RS26095 Protein ID WP_004150355.1
Coordinates 5330773..5331015 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTC85_RS26075 (5327756) 5327756..5328658 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
PTC85_RS26080 (5328655) 5328655..5329290 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
PTC85_RS26085 (5329287) 5329287..5330216 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
PTC85_RS26090 (5330390) 5330390..5330773 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
PTC85_RS26095 (5330773) 5330773..5331015 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
PTC85_RS26100 (5331220) 5331220..5332137 + 918 WP_021466676.1 alpha/beta hydrolase -
PTC85_RS26105 (5332151) 5332151..5333092 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
PTC85_RS26110 (5333137) 5333137..5333574 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
PTC85_RS26115 (5333571) 5333571..5334431 - 861 WP_004146232.1 virulence factor BrkB family protein -
PTC85_RS26120 (5334425) 5334425..5335024 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T272226 WP_002882817.1 NZ_CP117745:c5330773-5330390 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT272226 WP_004150355.1 NZ_CP117745:c5331015-5330773 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References