Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4736366..4737176 Replicon chromosome
Accession NZ_CP117745
Organism Klebsiella pneumoniae strain 2022CK-00752

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag PTC85_RS23290 Protein ID WP_002887280.1
Coordinates 4736366..4736899 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag PTC85_RS23295 Protein ID WP_002887278.1
Coordinates 4736910..4737176 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTC85_RS23285 (4735198) 4735198..4736319 + 1122 WP_009309849.1 cupin domain-containing protein -
PTC85_RS23290 (4736366) 4736366..4736899 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
PTC85_RS23295 (4736910) 4736910..4737176 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
PTC85_RS23300 (4737279) 4737279..4738712 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
PTC85_RS23305 (4738702) 4738702..4739385 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
PTC85_RS23310 (4739558) 4739558..4740943 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
PTC85_RS23315 (4740961) 4740961..4741266 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T272223 WP_002887280.1 NZ_CP117745:c4736899-4736366 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT272223 WP_002887278.1 NZ_CP117745:c4737176-4736910 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References