Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 142166..143076 Replicon plasmid unnamed1
Accession NZ_CP117741
Organism Klebsiella pneumoniae strain 2022CK-00768

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag PTC88_RS27255 Protein ID WP_004026354.1
Coordinates 142606..143076 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag PTC88_RS27250 Protein ID WP_004026357.1
Coordinates 142166..142609 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTC88_RS27235 (PTC88_27235) 138800..140287 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
PTC88_RS27240 (PTC88_27240) 140373..140795 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
PTC88_RS27245 (PTC88_27245) 140795..142066 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
PTC88_RS27250 (PTC88_27250) 142166..142609 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
PTC88_RS27255 (PTC88_27255) 142606..143076 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
PTC88_RS27265 (PTC88_27265) 144154..144606 + 453 Protein_154 CatA-like O-acetyltransferase -
PTC88_RS27270 (PTC88_27270) 144807..145184 - 378 WP_000656305.1 GNAT family N-acetyltransferase -
PTC88_RS27275 (PTC88_27275) 145495..146499 - 1005 WP_000427619.1 IS110-like element IS5075 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-5 / aph(3')-VI / qnrS1 / blaCTX-M-15 / mph(A) / sul1 / qacE / dfrA5 iucA / iucB / iucC / iucD / iutA / rmpA 1..341460 341460


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T272210 WP_004026354.1 NZ_CP117741:142606-143076 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT272210 WP_004026357.1 NZ_CP117741:142166-142609 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References