Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5322189..5322814 Replicon chromosome
Accession NZ_CP117740
Organism Klebsiella pneumoniae strain 2022CK-00768

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag PTC88_RS26065 Protein ID WP_002882817.1
Coordinates 5322189..5322572 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag PTC88_RS26070 Protein ID WP_004150355.1
Coordinates 5322572..5322814 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTC88_RS26050 (5319555) 5319555..5320457 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
PTC88_RS26055 (5320454) 5320454..5321089 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
PTC88_RS26060 (5321086) 5321086..5322015 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
PTC88_RS26065 (5322189) 5322189..5322572 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
PTC88_RS26070 (5322572) 5322572..5322814 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
PTC88_RS26075 (5323019) 5323019..5323936 + 918 WP_021466676.1 alpha/beta hydrolase -
PTC88_RS26080 (5323950) 5323950..5324891 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
PTC88_RS26085 (5324936) 5324936..5325373 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
PTC88_RS26090 (5325370) 5325370..5326230 - 861 WP_004146232.1 virulence factor BrkB family protein -
PTC88_RS26095 (5326224) 5326224..5326823 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T272208 WP_002882817.1 NZ_CP117740:c5322572-5322189 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT272208 WP_004150355.1 NZ_CP117740:c5322814-5322572 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References