Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 53024..53382 Replicon chromosome
Accession NC_017628
Organism Escherichia coli IHE3034

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag ECOK1_RS28695 Protein ID WP_001332330.1
Coordinates 53260..53382 (+) Length 41 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag ECOK1_RS00275 Protein ID WP_000125566.1
Coordinates 53024..53257 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOK1_RS00250 48097..48384 + 288 WP_000203741.1 ferredoxin-like protein FixX -
ECOK1_RS00255 48443..49774 + 1332 WP_001183207.1 MFS transporter -
ECOK1_RS00260 49882..50412 + 531 WP_000600749.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
ECOK1_RS00265 50405..52267 + 1863 WP_000377122.1 glutathione-regulated potassium-efflux system protein KefC -
ECOK1_RS00270 52459..52938 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
ECOK1_RS00275 53024..53257 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ECOK1_RS28695 53260..53382 + 123 WP_001332330.1 CcdB family protein Toxin
ECOK1_RS28700 53423..53575 + 153 WP_146692624.1 CcdB family protein -
ECOK1_RS00285 53572..54420 - 849 WP_000257201.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
ECOK1_RS00290 54427..54804 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
ECOK1_RS00295 54807..55628 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
ECOK1_RS00300 55625..56614 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
ECOK1_RS00305 56614..57900 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-39)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 41 a.a.        Molecular weight: 4563.34 Da        Isoelectric Point: 10.9266

>T27220 WP_001332330.1 NC_017628:53260-53382 [Escherichia coli IHE3034]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Download         Length: 123 bp

>T27220 NC_017628:53260-53382 [Escherichia coli IHE3034]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT27220 WP_000125566.1 NC_017628:53024-53257 [Escherichia coli IHE3034]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT27220 NC_017628:53024-53257 [Escherichia coli IHE3034]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References