Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 47307..47833 Replicon plasmid unnamed1
Accession NZ_CP117724
Organism Enterobacter asburiae strain 2022CK-00757

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag PTC87_RS22960 Protein ID WP_000323025.1
Coordinates 47307..47594 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag PTC87_RS22965 Protein ID WP_000534858.1
Coordinates 47594..47833 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTC87_RS22925 (42311) 42311..42664 - 354 WP_033147099.1 type IV conjugative transfer system pilin TraA -
PTC87_RS22930 (42861) 42861..43502 + 642 WP_032636186.1 Arc family DNA-binding protein -
PTC87_RS22935 (43535) 43535..43930 - 396 WP_023327730.1 hypothetical protein -
PTC87_RS22940 (44504) 44504..45043 + 540 WP_032636190.1 lytic transglycosylase domain-containing protein -
PTC87_RS22945 (45072) 45072..45893 - 822 WP_023327732.1 DUF932 domain-containing protein -
PTC87_RS22950 (45910) 45910..46203 - 294 WP_023327733.1 hypothetical protein -
PTC87_RS22955 (47081) 47081..47254 - 174 WP_233424125.1 DUF5431 family protein -
PTC87_RS22960 (47307) 47307..47594 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
PTC87_RS22965 (47594) 47594..47833 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
PTC87_RS22970 (47858) 47858..48163 + 306 WP_001326990.1 protein YdfV -
PTC87_RS22975 (48366) 48366..48698 + 333 WP_001317460.1 FlxA-like family protein -
PTC87_RS22980 (49135) 49135..50475 - 1341 WP_004196353.1 IS1202-like element ISEsa1 family transposase -
- (52070) 52070..52164 - 95 NuclAT_0 - -
- (52070) 52070..52164 - 95 NuclAT_0 - -
- (52070) 52070..52164 - 95 NuclAT_0 - -
- (52070) 52070..52164 - 95 NuclAT_0 - -
PTC87_RS22990 (52224) 52224..52598 - 375 WP_023327673.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..172688 172688


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T272175 WP_000323025.1 NZ_CP117724:c47594-47307 [Enterobacter asburiae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT272175 WP_000534858.1 NZ_CP117724:c47833-47594 [Enterobacter asburiae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References