Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 13419..13944 Replicon plasmid pAA
Accession NC_017627
Organism Escherichia coli 042

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag EC042_RS26315 Protein ID WP_001159871.1
Coordinates 13419..13724 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag EC042_RS26320 Protein ID WP_000813634.1
Coordinates 13726..13944 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC042_RS26290 9748..11286 - 1539 Protein_12 IS66 family transposase -
EC042_RS26295 11336..11683 - 348 WP_000612588.1 IS66 family insertion sequence element accessory protein TnpB -
EC042_RS30645 11680..12060 - 381 WP_001171558.1 IS66 family insertion sequence hypothetical protein -
EC042_RS30510 12221..12373 + 153 WP_144335553.1 helix-turn-helix domain-containing protein -
EC042_RS26310 12612..13418 - 807 WP_000016964.1 site-specific integrase -
EC042_RS26315 13419..13724 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
EC042_RS26320 13726..13944 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EC042_RS26325 14466..14725 + 260 Protein_19 hypothetical protein -
EC042_RS26330 14725..15315 + 591 Protein_20 hypothetical protein -
EC042_RS26335 15559..16401 + 843 WP_032489528.1 polysaccharide deacetylase family protein -
EC042_RS27190 16404..17492 + 1089 WP_011666408.1 putative hexosyltransferase CapU -
EC042_RS26345 17497..18447 + 951 WP_014639423.1 virulence factor VirK -
EC042_RS26350 18514..18774 + 261 Protein_24 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - aafB / aafC / afaB-I / pet / aafD / aafA / aap/aspU 1..113346 113346


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T27217 WP_001159871.1 NC_017627:c13724-13419 [Escherichia coli 042]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T27217 NC_017627:c13724-13419 [Escherichia coli 042]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT27217 WP_000813634.1 NC_017627:c13944-13726 [Escherichia coli 042]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT27217 NC_017627:c13944-13726 [Escherichia coli 042]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References