Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4821693..4822190 Replicon chromosome
Accession NC_017626
Organism Escherichia coli 042

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EC042_RS24005 Protein ID WP_001173343.1
Coordinates 4822017..4822190 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EC042_RS24000 Protein ID WP_000398619.1
Coordinates 4821693..4821989 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC042_RS23980 4818422..4819141 - 720 WP_000611277.1 two-component system response regulator DcuR -
EC042_RS23985 4819138..4820769 - 1632 WP_001216477.1 sensor histidine kinase -
EC042_RS23990 4820950..4821180 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
EC042_RS23995 4821192..4821464 + 273 WP_000405647.1 N-acetyltransferase -
EC042_RS24000 4821693..4821989 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EC042_RS24005 4822017..4822190 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EC042_RS24010 4822309..4823826 - 1518 WP_000003806.1 lysine--tRNA ligase -
EC042_RS24015 4824063..4825520 - 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T27213 WP_001173343.1 NC_017626:4822017-4822190 [Escherichia coli 042]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T27213 NC_017626:4822017-4822190 [Escherichia coli 042]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT27213 WP_000398619.1 NC_017626:4821693-4821989 [Escherichia coli 042]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT27213 NC_017626:4821693-4821989 [Escherichia coli 042]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References