Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 94890..96513 Replicon plasmid pB1S11-p2
Accession NZ_CP117719
Organism Escherichia coli strain B1S11

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag PTA69_RS25460 Protein ID WP_000088046.1
Coordinates 94890..96215 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag PTA69_RS25465 Protein ID WP_024136327.1
Coordinates 96220..96513 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTA69_RS25420 (PTA69_25420) 89911..90267 + 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -
PTA69_RS25425 (PTA69_25425) 90609..90812 - 204 WP_001194555.1 hypothetical protein -
PTA69_RS25430 (PTA69_25430) 90874..91731 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
PTA69_RS25435 (PTA69_25435) 91747..92055 - 309 WP_001224687.1 hypothetical protein -
PTA69_RS25440 (PTA69_25440) 92604..93029 - 426 WP_001043843.1 hypothetical protein -
PTA69_RS25445 (PTA69_25445) 93283..94098 - 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
PTA69_RS25450 (PTA69_25450) 94111..94521 - 411 WP_000985911.1 hypothetical protein -
PTA69_RS25455 (PTA69_25455) 94623..94829 - 207 WP_000134171.1 hypothetical protein -
PTA69_RS25460 (PTA69_25460) 94890..96215 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
PTA69_RS25465 (PTA69_25465) 96220..96513 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
PTA69_RS25470 (PTA69_25470) 97117..97509 - 393 WP_000341066.1 hypothetical protein -
PTA69_RS25475 (PTA69_25475) 97882..98223 + 342 WP_001151575.1 hypothetical protein -
PTA69_RS25480 (PTA69_25480) 98315..98506 + 192 WP_000398480.1 hypothetical protein -
PTA69_RS25485 (PTA69_25485) 98556..98837 + 282 WP_000077926.1 hypothetical protein -
PTA69_RS25490 (PTA69_25490) 99178..99309 - 132 WP_000374059.1 hypothetical protein -
PTA69_RS25495 (PTA69_25495) 99400..100641 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / ARR-3 / mph(A) / qnrS1 / blaCTX-M-55 / blaTEM-1B / rmtB / fosA3 / blaLAP-2 / aac(3)-IId / floR / aph(6)-Id / tet(A) / sul3 / lnu(F) / ant(3'')-Ia / aph(3')-Ia - 1..199026 199026


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T272129 WP_000088046.1 NZ_CP117719:c96215-94890 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT272129 WP_024136327.1 NZ_CP117719:c96513-96220 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References