Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 96734..97377 Replicon plasmid pPBS4
Accession NZ_CP117716
Organism Escherichia coli strain PBS4

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag PTA50_RS24720 Protein ID WP_001044768.1
Coordinates 96734..97150 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag PTA50_RS24725 Protein ID WP_001261287.1
Coordinates 97147..97377 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTA50_RS24705 (93233) 93233..93823 - 591 WP_000194575.1 hypothetical protein -
PTA50_RS24710 (93823) 93823..94080 - 258 WP_000343085.1 hypothetical protein -
PTA50_RS24715 (94434) 94434..96572 + 2139 WP_000350638.1 AAA family ATPase -
PTA50_RS24720 (96734) 96734..97150 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
PTA50_RS24725 (97147) 97147..97377 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
PTA50_RS24730 (97673) 97673..97963 + 291 WP_000111771.1 hypothetical protein -
PTA50_RS24735 (97953) 97953..98852 + 900 WP_000963206.1 nucleotide-binding protein -
PTA50_RS24740 (98902) 98902..101127 - 2226 WP_000698737.1 P-loop NTPase fold protein -
PTA50_RS24745 (101129) 101129..102217 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / floR / aph(3')-Ia / dfrA14 / blaCTX-M-55 / sitABCD iucA / iucB / iucC / iucD / iutA 1..137545 137545


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T272105 WP_001044768.1 NZ_CP117716:c97150-96734 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT272105 WP_001261287.1 NZ_CP117716:c97377-97147 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References