272091

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3556654..3557491 Replicon chromosome
Accession NZ_CP117714
Organism Escherichia coli strain PBS4

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag PTA50_RS17240 Protein ID WP_000227784.1
Coordinates 3556949..3557491 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag PTA50_RS17235 Protein ID WP_001297137.1
Coordinates 3556654..3556965 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTA50_RS17210 (3551674) 3551674..3552621 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
PTA50_RS17215 (3552643) 3552643..3554634 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
PTA50_RS17220 (3554624) 3554624..3555238 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
PTA50_RS17225 (3555238) 3555238..3555567 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
PTA50_RS17230 (3555579) 3555579..3556469 + 891 WP_000971336.1 heme o synthase -
PTA50_RS17235 (3556654) 3556654..3556965 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
PTA50_RS17240 (3556949) 3556949..3557491 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
PTA50_RS17245 (3557547) 3557547..3558482 - 936 WP_001297127.1 tetratricopeptide repeat protein -
PTA50_RS17250 (3558890) 3558890..3560254 + 1365 WP_001000975.1 MFS transporter -
PTA50_RS17255 (3560382) 3560382..3560873 - 492 WP_001138904.1 nucleotide binding protein YajQ -
PTA50_RS17260 (3561041) 3561041..3561952 + 912 WP_000705877.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T272091 WP_000227784.1 NZ_CP117714:3556949-3557491 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT272091 WP_001297137.1 NZ_CP117714:3556654-3556965 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References