Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 60878..61403 Replicon plasmid pEA4_88
Accession NZ_CP117680
Organism Escherichia albertii strain BIA_4

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag PS033_RS24420 Protein ID WP_001159871.1
Coordinates 60878..61183 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag PS033_RS24425 Protein ID WP_000813634.1
Coordinates 61185..61403 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PS033_RS24395 (56046) 56046..57612 - 1567 Protein_72 IS66 family transposase -
PS033_RS24400 (57632) 57632..57979 - 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
PS033_RS24405 (57979) 57979..58626 - 648 WP_059268691.1 IS66-like element accessory protein TnpA -
PS033_RS24410 (58907) 58907..59662 - 756 WP_000807692.1 replication initiation protein RepE -
PS033_RS24415 (60071) 60071..60877 - 807 WP_059268688.1 site-specific integrase -
PS033_RS24420 (60878) 60878..61183 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
PS033_RS24425 (61185) 61185..61403 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
PS033_RS24430 (62032) 62032..62262 + 231 WP_059268686.1 type II toxin-antitoxin system VapB family antitoxin -
PS033_RS24435 (62259) 62259..62675 + 417 WP_042093420.1 PIN domain-containing protein -
PS033_RS24440 (62845) 62845..64983 - 2139 WP_059268683.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..88280 88280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T272016 WP_001159871.1 NZ_CP117680:c61183-60878 [Escherichia albertii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT272016 WP_000813634.1 NZ_CP117680:c61403-61185 [Escherichia albertii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References