Detailed information of TA system TA027198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1797537..1798063
• Replicon: chromosome
• Accession: NC_017626
• Organism: Escherichia coli 042

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: EC042_RS09085
• Protein ID: WP_000323025.1
• Coordinates: 1797537..1797824 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: EC042_RS09090
• Protein ID: WP_000534858.1
• Coordinates: 1797824..1798063 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC042_RS09045	1792561..1792776	-	216	WP_000839590.1	phage lysis protein EssD	-
EC042_RS09050	1793530..1793745	-	216	WP_000066484.1	cold shock-like protein CspB	-
EC042_RS09055	1794046..1794258	+	213	WP_000087756.1	cold shock-like protein CspF	-
EC042_RS30120	1794313..1794402	+	90	WP_120795389.1	hypothetical protein	-
EC042_RS09060	1794680..1795432	-	753	WP_001047135.1	antitermination protein	-
EC042_RS09065	1795446..1796495	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
EC042_RS09070	1796497..1796775	-	279	WP_012304870.1	hypothetical protein	-
EC042_RS09075	1796842..1797093	-	252	WP_000980994.1	hypothetical protein	-
EC042_RS09080	1797310..1797465	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
EC042_RS09085	1797537..1797824	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
EC042_RS09090	1797824..1798063	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
EC042_RS27845	1798088..1798393	+	306	WP_001326990.1	hypothetical protein	-
EC042_RS09095	1798737..1798928	+	192	WP_000211628.1	FlxA-like family protein	-
EC042_RS09100	1799365..1800678	-	1314	WP_000589008.1	ISNCY family transposase	-
EC042_RS30930	1801447..1801734	-	288	Protein_1726	hypothetical protein	-
EC042_RS09115	1802345..1802701	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1755914..1811310	55396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T27198 WP_000323025.1 NC_017626:c1797824-1797537 [Escherichia coli 042]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T27198 NC_017626:c1797824-1797537 [Escherichia coli 042]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT27198 WP_000534858.1 NC_017626:c1798063-1797824 [Escherichia coli 042]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT27198 NC_017626:c1798063-1797824 [Escherichia coli 042]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8