Detailed information of TA system TA027196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1633639..1634277
• Replicon: chromosome
• Accession: NC_017626
• Organism: Escherichia coli 042

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D3GRG9
• Locus tag: EC042_RS08280
• Protein ID: WP_001314712.1
• Coordinates: 1633639..1633815 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EC042_RS08285
• Protein ID: WP_077248684.1
• Coordinates: 1633861..1634277 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC042_RS08250	1629090..1629626	+	537	WP_000429149.1	DNA-binding transcriptional regulator SutR	-
EC042_RS08255	1629699..1631660	+	1962	WP_001314711.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EC042_RS08275	1633187..1633417	-	231	WP_000494241.1	YncJ family protein	-
EC042_RS08280	1633639..1633815	+	177	WP_001314712.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EC042_RS08285	1633861..1634277	+	417	WP_077248684.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EC042_RS08290	1634356..1635762	+	1407	WP_000760623.1	PLP-dependent aminotransferase family protein	-
EC042_RS08295	1636007..1637152	+	1146	WP_000047442.1	ABC transporter substrate-binding protein	-
EC042_RS08300	1637170..1638183	+	1014	WP_000220407.1	ABC transporter ATP-binding protein	-
EC042_RS08305	1638184..1639125	+	942	WP_001251335.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1632589..1642920	10331

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (87-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6746.81 Da
• Isoelectric Point: 11.8888

>T27196 WP_001314712.1 NC_017626:1633639-1633815 [Escherichia coli 042]
VKQSEFRRWLESQGVNVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T27196 NC_017626:1633639-1633815 [Escherichia coli 042]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCAATGTAGCAAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTACGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.60 Da
• Isoelectric Point: 4.7286

>AT27196 WP_077248684.1 NC_017626:1633861-1634277 [Escherichia coli 042]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSDITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT27196 NC_017626:1633861-1634277 [Escherichia coli 042]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGACATCACTCAGCAAGAATTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: D3GRG9