Detailed information of TA system TA027186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 989159..989864
• Replicon: chromosome
• Accession: NC_017626
• Organism: Escherichia coli 042

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D3GZQ4
• Locus tag: EC042_RS04850
• Protein ID: WP_000539523.1
• Coordinates: 989478..989864 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EC042_RS04845
• Protein ID: WP_001280945.1
• Coordinates: 989159..989488 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC042_RS04830	984316..985227	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
EC042_RS04835	985405..987753	+	2349	WP_014639116.1	EAL domain-containing protein	-
EC042_RS04840	987761..989089	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
EC042_RS04845	989159..989488	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
EC042_RS04850	989478..989864	-	387	WP_000539523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EC042_RS04855	990089..991414	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
EC042_RS04860	991627..992010	+	384	WP_000497137.1	biofilm formation regulator BssR	-
EC042_RS04865	992121..993236	+	1116	WP_000554967.1	aldose sugar dehydrogenase YliI	-
EC042_RS04870	993233..993859	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.50 Da
• Isoelectric Point: 9.9296

>T27186 WP_000539523.1 NC_017626:c989864-989478 [Escherichia coli 042]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELVAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T27186 NC_017626:c989864-989478 [Escherichia coli 042]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGTGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT27186 WP_001280945.1 NC_017626:c989488-989159 [Escherichia coli 042]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT27186 NC_017626:c989488-989159 [Escherichia coli 042]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGACCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: D3GZQ4