Detailed information of TA system TA027182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 56935..57485
• Replicon: chromosome
• Accession: NC_017626
• Organism: Escherichia coli 042

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: EC042_RS00300
• Protein ID: WP_001160975.1
• Coordinates: 57171..57485 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D3H431
• Locus tag: EC042_RS00295
• Protein ID: WP_000125565.1
• Coordinates: 56935..57168 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC042_RS00270	52005..52292	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
EC042_RS00275	52351..53682	+	1332	WP_001183207.1	MFS transporter	-
EC042_RS00280	53793..54323	+	531	WP_000600718.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
EC042_RS00285	54316..56178	+	1863	WP_000377186.1	glutathione-regulated potassium-efflux system protein KefC	-
EC042_RS00290	56370..56849	+	480	WP_001487721.1	type 3 dihydrofolate reductase	-
EC042_RS00295	56935..57168	+	234	WP_000125565.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
EC042_RS00300	57171..57485	+	315	WP_001160975.1	CcdB family protein	Toxin
EC042_RS00305	57482..58330	-	849	WP_000257185.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
EC042_RS00310	58337..58714	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
EC042_RS00315	58717..59538	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
EC042_RS00320	59535..60524	-	990	WP_000241272.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
EC042_RS00325	60524..61810	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T27182 WP_001160975.1 NC_017626:57171-57485 [Escherichia coli 042]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T27182 NC_017626:57171-57485 [Escherichia coli 042]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8599.64 Da
• Isoelectric Point: 6.9791

>AT27182 WP_000125565.1 NC_017626:56935-57168 [Escherichia coli 042]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Nucleotide

• Length: 234 bp

>AT27182 NC_017626:56935-57168 [Escherichia coli 042]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGCGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A090NGC7