Detailed information of TA system TA027174

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3608147..3608826
• Replicon: chromosome
• Accession: NC_017625
• Organism: Escherichia coli DH1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: ECDH1_RS17885
• Protein ID: WP_000854672.1
• Coordinates: 3608485..3608826 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: ECDH1_RS17880
• Protein ID: WP_000070395.1
• Coordinates: 3608147..3608464 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECDH1_RS17840	3603194..3604057	+	864	WP_001065553.1	GTPase family protein	-
ECDH1_RS17845	3604149..3604970	+	822	WP_000197389.1	DUF945 domain-containing protein	-
ECDH1_RS25820	3605187..3605378	+	192	Protein_3403	DeoR family transcriptional regulator	-
ECDH1_RS25825	3605374..3605601	+	228	WP_001548158.1	hypothetical protein	-
ECDH1_RS25830	3605601..3606044	+	444	WP_000824223.1	hypothetical protein	-
ECDH1_RS17860	3606067..3606534	+	468	WP_001547765.1	hypothetical protein	-
ECDH1_RS17865	3606611..3606850	+	240	WP_000194654.1	DUF905 domain-containing protein	-
ECDH1_RS17870	3606948..3607406	+	459	WP_000211838.1	antirestriction protein	-
ECDH1_RS17875	3607422..3607898	+	477	WP_000811693.1	RadC family protein	-
ECDH1_RS24505	3607907..3608128	+	222	WP_000691994.1	DUF987 domain-containing protein	-
ECDH1_RS17880	3608147..3608464	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
ECDH1_RS17885	3608485..3608826	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
ECDH1_RS17895	3609398..3610651	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
ECDH1_RS17900	3610663..3611766	-	1104	WP_001285288.1	glutamate 5-kinase	-
ECDH1_RS17905	3612054..3613109	+	1056	WP_000749863.1	phosphoporin PhoE	-
ECDH1_RS17910	3613148..3613549	-	402	WP_000174689.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T27174 WP_000854672.1 NC_017625:3608485-3608826 [Escherichia coli DH1]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T27174 NC_017625:3608485-3608826 [Escherichia coli DH1]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT27174 WP_000070395.1 NC_017625:3608147-3608464 [Escherichia coli DH1]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT27174 NC_017625:3608147-3608464 [Escherichia coli DH1]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684