Detailed information of TA system TA027173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3390247..3390865
• Replicon: chromosome
• Accession: NC_017625
• Organism: Escherichia coli DH1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: ECDH1_RS16775
• Protein ID: WP_001290581.1
• Coordinates: 3390647..3390865 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: ECDH1_RS16770
• Protein ID: WP_000344800.1
• Coordinates: 3390247..3390621 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECDH1_RS16760	3385336..3386529	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
ECDH1_RS16765	3386552..3389701	+	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
ECDH1_RS16770	3390247..3390621	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
ECDH1_RS16775	3390647..3390865	+	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
ECDH1_RS16780	3391037..3391588	+	552	WP_000102564.1	maltose O-acetyltransferase	-
ECDH1_RS16785	3391704..3392174	+	471	WP_000136192.1	YlaC family protein	-
ECDH1_RS16790	3392338..3393888	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
ECDH1_RS16795	3393930..3394283	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
ECDH1_RS16800	3394662..3394973	+	312	WP_000409911.1	MGMT family protein	-
ECDH1_RS16805	3395004..3395576	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T27173 WP_001290581.1 NC_017625:3390647-3390865 [Escherichia coli DH1]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T27173 NC_017625:3390647-3390865 [Escherichia coli DH1]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT27173 WP_000344800.1 NC_017625:3390247-3390621 [Escherichia coli DH1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT27173 NC_017625:3390247-3390621 [Escherichia coli DH1]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5