Detailed information of TA system TA027172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2368554..2369192
• Replicon: chromosome
• Accession: NC_017625
• Organism: Escherichia coli DH1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECDH1_RS11680
• Protein ID: WP_000813794.1
• Coordinates: 2369016..2369192 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECDH1_RS11675
• Protein ID: WP_001270286.1
• Coordinates: 2368554..2368970 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECDH1_RS11655	2363706..2364647	-	942	WP_001251304.1	ABC transporter permease	-
ECDH1_RS11660	2364648..2365661	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
ECDH1_RS11665	2365679..2366824	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
ECDH1_RS11670	2367069..2368475	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
ECDH1_RS11675	2368554..2368970	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECDH1_RS11680	2369016..2369192	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECDH1_RS11685	2369414..2369644	+	231	WP_000494244.1	YncJ family protein	-
ECDH1_RS11690	2369736..2371697	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECDH1_RS11695	2371770..2372306	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECDH1_RS11700	2372359..2373573	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T27172 WP_000813794.1 NC_017625:c2369192-2369016 [Escherichia coli DH1]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T27172 NC_017625:c2369192-2369016 [Escherichia coli DH1]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT27172 WP_001270286.1 NC_017625:c2368970-2368554 [Escherichia coli DH1]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT27172 NC_017625:c2368970-2368554 [Escherichia coli DH1]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB