Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 29688..30213 Replicon plasmid pEA8_1
Accession NZ_CP117559
Organism Escherichia albertii strain BIA_8

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag PS052_RS23325 Protein ID WP_001159868.1
Coordinates 29908..30213 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag PS052_RS23320 Protein ID WP_000813634.1
Coordinates 29688..29906 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PS052_RS23290 (25014) 25014..25231 + 218 Protein_19 transposase -
PS052_RS23295 (25287) 25287..25603 - 317 Protein_20 type II toxin-antitoxin system VapC family toxin -
PS052_RS23300 (25790) 25790..26977 - 1188 WP_273765785.1 IS91 family transposase -
PS052_RS23305 (26977) 26977..27273 - 297 WP_059225599.1 hypothetical protein -
PS052_RS23310 (27432) 27432..27659 - 228 Protein_23 type II toxin-antitoxin system VapB family antitoxin -
PS052_RS23315 (27858) 27858..29207 - 1350 WP_243043965.1 hypothetical protein -
PS052_RS23320 (29688) 29688..29906 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
PS052_RS23325 (29908) 29908..30213 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
PS052_RS23330 (30214) 30214..30357 + 144 Protein_27 hypothetical protein -
PS052_RS23335 (30457) 30457..31629 + 1173 WP_053264206.1 IS21-like element IS21 family transposase -
PS052_RS23340 (31629) 31629..32426 + 798 WP_273764657.1 IS21-like element IS21 family helper ATPase IstB -
PS052_RS23345 (32486) 32486..33154 + 669 Protein_30 site-specific integrase -
PS052_RS23350 (33876) 33876..34631 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..108071 108071
- inside IScluster/Tn - - 14528..32426 17898


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T271383 WP_001159868.1 NZ_CP117559:29908-30213 [Escherichia albertii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT271383 WP_000813634.1 NZ_CP117559:29688-29906 [Escherichia albertii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References