Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 24210..25013 Replicon plasmid pEA8_1
Accession NZ_CP117559
Organism Escherichia albertii strain BIA_8

Toxin (Protein)


Gene name KacT Uniprot ID A0A8S7IG51
Locus tag PS052_RS23285 Protein ID WP_053285705.1
Coordinates 24483..25013 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag PS052_RS23280 Protein ID WP_001275013.1
Coordinates 24210..24479 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PS052_RS23255 (19624) 19624..20020 + 397 Protein_12 IS481 family transposase -
PS052_RS23260 (20526) 20526..21161 + 636 WP_074458670.1 T3SS effector NleG family protein -
PS052_RS23265 (21299) 21299..21635 + 337 Protein_14 IS630 family transposase -
PS052_RS23270 (21718) 21718..22083 + 366 WP_024223721.1 hypothetical protein -
PS052_RS23275 (22083) 22083..23270 + 1188 WP_187183929.1 IS91 family transposase -
PS052_RS23280 (24210) 24210..24479 + 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
PS052_RS23285 (24483) 24483..25013 + 531 WP_053285705.1 GNAT family N-acetyltransferase Toxin
PS052_RS23290 (25014) 25014..25231 + 218 Protein_19 transposase -
PS052_RS23295 (25287) 25287..25603 - 317 Protein_20 type II toxin-antitoxin system VapC family toxin -
PS052_RS23300 (25790) 25790..26977 - 1188 WP_273765785.1 IS91 family transposase -
PS052_RS23305 (26977) 26977..27273 - 297 WP_059225599.1 hypothetical protein -
PS052_RS23310 (27432) 27432..27659 - 228 Protein_23 type II toxin-antitoxin system VapB family antitoxin -
PS052_RS23315 (27858) 27858..29207 - 1350 WP_243043965.1 hypothetical protein -
PS052_RS23320 (29688) 29688..29906 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..108071 108071
- inside IScluster/Tn - - 14528..32426 17898


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-155)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20291.22 Da        Isoelectric Point: 6.7483

>T271382 WP_053285705.1 NZ_CP117559:24483-25013 [Escherichia albertii]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKVENCDSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT271382 WP_001275013.1 NZ_CP117559:24210-24479 [Escherichia albertii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References