Detailed information of TA system TA271316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1656239..1657934
• Replicon: chromosome
• Accession: NZ_CP117527
• Organism: Pseudomonas aeruginosa strain MF1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: PSP57_RS07560
• Protein ID: WP_023095293.1
• Coordinates: 1656239..1657108 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: PSP57_RS07565
• Protein ID: WP_003112902.1
• Coordinates: 1657101..1657934 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSP57_RS07535 (PSP57_07535)	1652247..1653098	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
PSP57_RS07540 (PSP57_07540)	1653139..1654146	+	1008	WP_022579704.1	ABC transporter substrate-binding protein	-
PSP57_RS07545 (PSP57_07545)	1654143..1654919	+	777	WP_003112898.1	ABC transporter permease	-
PSP57_RS07550 (PSP57_07550)	1654925..1655686	+	762	WP_003112899.1	SDR family oxidoreductase	-
PSP57_RS07555 (PSP57_07555)	1655700..1656230	+	531	WP_003112900.1	cupin domain-containing protein	-
PSP57_RS07560 (PSP57_07560)	1656239..1657108	+	870	WP_023095293.1	alpha/beta hydrolase	Toxin
PSP57_RS07565 (PSP57_07565)	1657101..1657934	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
PSP57_RS07570 (PSP57_07570)	1657921..1658718	+	798	WP_022579702.1	SDR family oxidoreductase	-
PSP57_RS07575 (PSP57_07575)	1658711..1660393	+	1683	WP_003112904.1	thiamine pyrophosphate-binding protein	-
PSP57_RS07580 (PSP57_07580)	1660404..1661207	+	804	WP_003112905.1	aspartate dehydrogenase	-
PSP57_RS07585 (PSP57_07585)	1661219..1662703	+	1485	WP_022579701.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30379.61 Da
• Isoelectric Point: 6.8139

>T271316 WP_023095293.1 NZ_CP117527:1656239-1657108 [Pseudomonas aeruginosa]
MNMAQTHIPAPSLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAARLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERLGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSPISGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT271316 WP_003112902.1 NZ_CP117527:1657101-1657934 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

Structures

Antitoxin

• AlphaFold DB: Q9HYA1