271308

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 56258..57395 Replicon plasmid pNBZ135-optrA-105K
Accession NZ_CP117512
Organism Enterococcus faecalis strain CQNBZ21-135

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag PSC79_RS13740 Protein ID WP_128704137.1
Coordinates 56532..57395 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag PSC79_RS13735 Protein ID WP_002326825.1
Coordinates 56258..56530 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSC79_RS13700 (PSC79_13735) 51576..52118 + 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
PSC79_RS13705 (PSC79_13740) 52211..53005 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
PSC79_RS13710 (PSC79_13745) 53574..53642 + 69 WP_273946095.1 23S rRNA methyltransferase attenuation leader peptide -
PSC79_RS13715 (PSC79_13750) 53767..54504 + 738 WP_181710332.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
PSC79_RS13720 (PSC79_13755) 54674..54934 + 261 Protein_61 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
PSC79_RS13725 (PSC79_13760) 55037..55933 + 897 WP_002326827.1 ParA family protein -
PSC79_RS13730 (PSC79_13765) 56025..56240 + 216 WP_001835296.1 peptide-binding protein -
PSC79_RS13735 (PSC79_13770) 56258..56530 + 273 WP_002326825.1 antitoxin Antitoxin
PSC79_RS13740 (PSC79_13775) 56532..57395 + 864 WP_128704137.1 zeta toxin family protein Toxin
PSC79_RS13745 (PSC79_13780) 57835..58152 + 318 WP_002326830.1 hypothetical protein -
PSC79_RS13750 (PSC79_13785) 58449..58955 + 507 WP_002415429.1 trimethoprim-resistant dihydrofolate reductase DfrG -
PSC79_RS13755 (PSC79_13790) 58989..59669 - 681 WP_010710189.1 IS6-like element IS1216 family transposase -
PSC79_RS13760 (PSC79_13795) 59744..61372 + 1629 Protein_69 ArdC-like ssDNA-binding domain-containing protein -
PSC79_RS13765 (PSC79_13800) 61405..61677 + 273 WP_002362409.1 LtrD -
PSC79_RS13770 (PSC79_13805) 61923..62279 + 357 WP_002362408.1 plasmid mobilization relaxosome protein MobC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cat / tet(L) / tet(M) / fexA / optrA / erm(A) / ant(6)-Ia / aph(3')-III / erm(B) / dfrG - 1..105647 105647
- inside IScluster/Tn fexA / optrA / erm(A) / ant(6)-Ia / aph(3')-III / erm(B) / dfrG - 31367..59675 28308


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32703.33 Da        Isoelectric Point: 7.3939

>T271308 WP_128704137.1 NZ_CP117512:56532-57395 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMVVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT271308 WP_002326825.1 NZ_CP117512:56258-56530 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References