Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 431265..432402 Replicon chromosome
Accession NZ_CP117508
Organism Enterococcus faecalis strain CQJXZ21-077

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag PSC78_RS02200 Protein ID WP_024417198.1
Coordinates 431539..432402 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag PSC78_RS02195 Protein ID WP_000301765.1
Coordinates 431265..431537 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSC78_RS02160 (426786) 426786..428159 + 1374 Protein_402 DNA topoisomerase -
PSC78_RS02165 (428293) 428293..428532 + 240 WP_002425012.1 peptide-binding protein -
PSC78_RS02170 (428596) 428596..428664 + 69 WP_011100846.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
PSC78_RS02175 (428789) 428789..429526 + 738 WP_032495453.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
PSC78_RS02180 (429696) 429696..429956 + 261 Protein_406 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
PSC78_RS02185 (430038) 430038..430934 + 897 WP_002387620.1 ParA family protein -
PSC78_RS02190 (431033) 431033..431248 + 216 WP_273961499.1 peptide-binding protein -
PSC78_RS02195 (431265) 431265..431537 + 273 WP_000301765.1 antitoxin Antitoxin
PSC78_RS02200 (431539) 431539..432402 + 864 WP_024417198.1 zeta toxin family protein Toxin
PSC78_RS02205 (432843) 432843..433160 + 318 WP_002338433.1 hypothetical protein -
PSC78_RS02210 (433694) 433694..434191 + 498 WP_010713926.1 molecular chaperone DnaJ -
PSC78_RS02215 (434226) 434226..434348 + 123 WP_159111623.1 DpnD/PcfM family protein -
PSC78_RS02220 (434402) 434402..435082 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
PSC78_RS02225 (435133) 435133..435576 - 444 Protein_415 MFS transporter -
PSC78_RS02230 (435777) 435777..436187 - 411 WP_231428140.1 protein rep -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn erm(B) / tet(O/W/32/O) / fexB - 425493..443304 17811


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T271266 WP_024417198.1 NZ_CP117508:431539-432402 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT271266 WP_000301765.1 NZ_CP117508:431265-431537 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References