Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 24957..25693 Replicon plasmid unnamed3
Accession NZ_CP117484
Organism Klebsiella pneumoniae strain DS-1

Toxin (Protein)


Gene name tacT Uniprot ID J5UJ49
Locus tag PPH92_RS27445 Protein ID WP_009654334.1
Coordinates 24957..25439 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag PPH92_RS27450 Protein ID WP_003026799.1
Coordinates 25427..25693 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPH92_RS27435 (PPH92_27435) 20400..23369 + 2970 WP_024945956.1 Tn3-like element TnShfr1 family transposase -
PPH92_RS27440 (PPH92_27440) 23448..24452 + 1005 WP_000427620.1 IS110-like element IS4321 family transposase -
PPH92_RS27445 (PPH92_27445) 24957..25439 - 483 WP_009654334.1 GNAT family N-acetyltransferase Toxin
PPH92_RS27450 (PPH92_27450) 25427..25693 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
PPH92_RS27455 (PPH92_27455) 25932..26366 - 435 WP_042928448.1 cell envelope integrity protein TolA -
PPH92_RS27460 (PPH92_27460) 26427..27395 + 969 WP_077266288.1 IS5 family transposase -
PPH92_RS27465 (PPH92_27465) 27441..28295 - 855 WP_273857275.1 cysteine peptidase family C39 domain-containing protein -
PPH92_RS27470 (PPH92_27470) 28285..29562 - 1278 WP_009309915.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..85339 85339
- inside IScluster/Tn - - 14777..27395 12618


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17339.06 Da        Isoelectric Point: 8.7400

>T271243 WP_009654334.1 NZ_CP117484:c25439-24957 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHVKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYVHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT271243 WP_003026799.1 NZ_CP117484:c25693-25427 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4QCA1

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References